1. The characterization of genetic backgrounds is favorable to select good near-isogenic lines. 对近等基因系进行遗传背景检测，有利于选育高质量的近等基因系材料。
2. The near-isogenic lines (NILs) are the important materials in studying the performance and function of the crop traits. 近等基因系是研究作物性状表现与功能的重要材料。
3. The near isogenic lines with Oleson did not affect the spikelet numbers and flag leaf length of backcrossing male parents. 奥尔森矮源近等基因系对回交父本的小穗数没有影响，对旗叶长影响比较小；
4. Two PCR amplified fragments were found to be tightly linked to Pi-h-1 (t) by RAPD analysis. of near isogenic pools of DNA from F2 plants. 应用近等基因池DNA和随机引物，经PCR扩增和共分离分析，建立了二个RAPD片段与抗病基因紧密连锁。
5. Near isogenic lines were important base for the construction of molecular genetic maps, QTLs location and molecular markers-assisted breeding. 近等基因系是分子遗传图谱构建、数量性状基因定位及分子标记辅助育种的重要基础。
